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KMID : 0613820140240121364
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Journal of Life Science
2014 Volume.24 No. 12 p.1364 ~ p.1370
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Effect of Ascorbic Acid on the Gravitropic Response of Primary Roots in Maize
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Kim Chung-Su
Mulkey Timothy J.
Kim Soon-Young
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Abstract
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Ascorbic acid (AA) is a multifunctional metabolite in plants that is essential for plant development and growth. We examined the effect of AA, an antioxidant, on the gravitropic response of primary roots in maize. The application of 10-3 M AA to the elongation zone did not affect the gravitropic response and slightly inhibited the root growth. However, treatment with both 10-5 M and 10-3 M AA at the root tip increased the gravitropic response and inhibited root growth. Differences in indole-3- acetic acid (IAA) activity between the upper and lower hemispheres of the root resulted in differential elongation along the horizontal root. Roots are extremely sensitive to IAA, and increasing the amount of IAA in the lower hemisphere of the root inhibited elongation. Therefore, we examined the effect of IAA in the presence of AA. The inhibitory effect of AA on the gravitropic response was greater in combination with IAA. To understand the role of AA in the regulation of root growth and the gravitropic response, we measured ethylene production in the presence of AA in the primary roots of maize. AA stimulated ethylene production via the activation of the 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase gene, which regulates the conversion of ACC to ethylene. These results suggest that AA alters the gravitropic response of maize roots through modification of the action of ethylene.
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KEYWORD
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Ascorbic acid (AA), ACC oxidase, ethylene, gravitropic response
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